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Multiparametric investigation of cardiac physiology is crucial for the diagnosis and therapy of heart disease. However, no method exists to simultaneously map multiple parameters that govern cardiac (patho)physiology from beating hearts in vivo. Here, we present a cardiac sensing platform that addresses this challenge, functioning with a wireless interface. Advanced fabrication and assembling strategies enable the heterogeneous integration of transparent microelectrodes, light-emitting diodes, photodiodes, and optical filters into a multilayer array structure on soft substrates. The microelectrodes exhibit superior electrochemical performance for measuring electrical potentials and excellent transparency for co-localized fluorescence measurement. The device shows excellent biocompatibility and records the fluorescence of calcium reporter with performance comparable to imaging cameras. Multiparametric in vivo mapping of electrical excitation, calcium dynamics, and their combined effects on cardiac excitation-contraction coupling is demonstrated during normal rhythm, arrhythmia, and treatment. This technology offers potential widespread use in cardiac research to support scientific discoveries and advance clinical life-saving diagnostics and therapies. 

Transparent microelectrode arrays (MEAs) that allow multimodal investigation of the spatiotemporal cardiac characteristics are important in studying and treating heart disease. Existing implantable devices, however, are designed to support chronic operational lifetimes and require surgical extraction when they malfunction or are no longer needed. Meanwhile, bioresorbable systems that can self-eliminate after performing temporary functions are increasingly attractive because they avoid the costs/risks of surgical extraction. We report the design, fabrication, characterization, and validation of a soft, fully bioresorbable, and transparent MEA platform for bidirectional cardiac interfacing over a clinically relevant period. The MEA provides multiparametric electrical/optical mapping of cardiac dynamics and on-demand site-specific pacing to investigate and treat cardiac dysfunctions in rat and human heart models. The bioresorption dynamics and biocompatibility are investigated. The device designs serve as the basis for bioresorbable cardiac technologies for potential postsurgical monitoring and treating temporary patient pathological conditions in certain clinical scenarios, such as myocardial infarction, ischemia, and transcatheter aortic valve replacement.